Chromatography Essay examples -- essays research papers

Chromatography is a separation technique in which the alloy to be separated is dissolved in a solvent and the resulting solution, often called the mobile bod, is then passed through or over another material, the nonmoving phase. The separation of the original potpourri depends on how strongly each gene is attracted to the stationary phase. Substances that are attracted strongly to the stationary phase allow be retarded and not move alone with the mobile phase. Weakly attracted substances will move more rapidly with the mobile phase.Liquid chromatography is an analytical technique that is useful for separating ions or molecules that are dissolved in a pellucid phase. If the sample solution is in contact with a second solidity or liquid phase, the different solutes will interact with the other phase to differing degrees due to differences in adsorption, ionic strength, polarity or size. These differences allow the mixture components to be separated from each other by using t hese differences to moderate the transit time of the solutes through a column.Simple liquid chromatography consists of a column with a fritted bottom that holds a stationary phase in equilibrium with a solvent. Typical stationary phases (and their interactions with solutes) are solids (adsorption), ionic groups on a resin (ion-exchange), liquids on an inert solid support (partitioning), and permeable inert particles (size exclusion). The mixtur...